What is the standard workflow for a gene expression experiment?
After an initial meeting to discuss the project goals, experimental design, and logistics, our users deliver isolated totalRNA samples for each of their experimental conditions. We then check the RNA quality and concentration in the Agilent 2100 BioAnalyzer prior to ordering the expression arrays. If everything checks out, we proceed with the amplification, fragmentation, and labeling reactions. Next, a spectrophotometer reading on each sample tells us whether or not the amplification was successful, and if so, the samples are hybridized to the array. Following the overnight hybridization the arrays are scanned, and the raw data extracted. Upon completion of the experiment, the raw expression data is made available to our users, or we will assist them with the analysis. Note: In the rare event that any of aforementioned reactions fail either fully or partially, users are contacted in order to resolve the issues before proceeding with the hybridization.
