What is the smallest experiment I can run?
Two arrays. If all you are looking for is basic data about differential gene expression, it is possible to run two arrays using pooled samples of total RNA. For an experimental this small,we prefer to run a reference design, combining our reference RNA labeled with Cy-3 with your samples labeled with Cy-5. One array will be hybridized with your pooled control (plus ref); the other with pooled exposed sample (plus ref). The data from the two arrays is analyzed to reveal genes that are differentially expressed. Bear in mind that the sample size for each group is n=1, and this is not likely to give you enough support for publication. Normally, the minimum order is 6 arrays, but contact us – we may be able to authorize fewer arrays.
