What is the significance of the melt curve analysis?
The melt curve analysis is performed as the final step in each assay as a way to detect non-specific products. These products can result from spurious amplification (due to non-specific priming) and/or the formation of primer/dimers. This analysis is a crucial step when using an intercalating dye such as SYBR Green. Q: What is the turn-around time for performing a new assay ? A: Once primers, the reference sample and RNA samples arrive to the VBI CLF, it typically takes seven business days to perform the needed optimization reactions. The assay is performed in a 96-well format. Data generation is dependent on the number of samples and genes to be assayed.
