What is the sensitivity of Threshold System assays?
The sensitivity depends on the analyte being measured. For total DNA, using the Total DNA Assay Kit, the limit of detection is 2 picograms. When measuring contaminant proteins using the Immuno-Ligand Assay Kit, the limit of detection will be dependent upon the antibodies used. Molecular Devices has prepared Applications Notes for many of the common contaminants. As examples, the limit of detection for BSA is 125 pg/mL, for bovine IgG it is 50 pg/mL, and for Protein A it is 20 pg/mL (in the presence of MuIgG1, the limit of detection is 25 ppb). Using the Immuno-Ligand Assay Kit and a model system developed for measuring the E. coli plasmid pGEM3, the limit of detection is 400 attomoles (which is equivalent to 750 pg or 235 x 106 molecules). For each specific analyte, the limit of detection is dependent upon the binding proteins, or DNA probes, and the assay configuration selected. However, the Threshold system will allow the maximum sensitivity with the available reagents, without the u
