What is the sensitivity of Krypton Protein Stain?
The stain is sensitive down to 0.25 ng using the standard protocol, or down to 2 ng using the rapid 30 minute protocol. A broad range of proteins have been tested with molecular weights from 14.4 to 200 K, including glycoproteins and phosphoproteins. Some of the proteins tested are as follows: myosin, beta-galactosidase, phosphorylase B, bovine serum albumin, ovalbumin, carbonic anhydrase, soybean trypsin inhibitor, lysozyme, aprotinin, horseradish peroxidase, myokinase, avidin, glucose oxidase, a2-macroglobulin, a1-acid glycoprotein, and beta-casein. (HeLa cell and rat heart tissue lysates were tested with 2D applications).
