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What is the sensitivity of 2D DIGE? How many protein spots can you detect in the 2D DIGE experiment?

April 26, 2017detect dige experiment protein spots

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What is the sensitivity of 2D DIGE? How many protein spots can you detect in the 2D DIGE experiment?

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The sensitivity of 2D DIGE is about 0.2 ng/spot. In general, using a pH 3-10 IPG strip, we will be able to detect over 2000 protein spots. If additional IPG strips were run using pH 4-7, pH 6-9 and pH 7-11, you will be able to detect over 5000 spots.