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What is the sample to working reagent ratio?

April 26, 2017ratio Reagent sample working

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What is the sample to working reagent ratio?

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For the Microplate Protocol the sample to working reagent (WR) ratio is 1:8 (25µl sample plus 200 ul WR). If the sample amount is limited, a 1:20 ratio may be used (10 ul sample); in this case, however, the detection range of the assay will be limited to 125-2,000µg/ml. For the Test Tube Procedure (Standard or Enhanced Protocols) the ratio is 1:20 (0.1 ml sample plus 2.0 ml WR). In the Micro BCA Assay Kit (for both Microplate and Test Tube Procedures), the ratio is 1:1 (1 part sample plus 1 part WR).