What is the role of specificity in target-specific selection?
In target-specific selection for sequencing, often the goal is target sequence enrichment. Frequently it is more important to capture the highest possible percentage of the sequences of interest while sacrificing non-specific capture. In comparison, microarray hybridization requires high specificity for hybridization probes in order to obtain accurate signal reading for the genes analyzed. Through capture probe design, the degree of specificity can be varied according to experimental requirements. Capture probes can be designed to be as specific as microarray probes when this is required.
