What is the required biosafety level for using recombinant adenovirus?
The recombinant adenoviruses we made are replication deficient due to deletions in the E1 and E3 regions. According to references issued by the NIH Office of Biosafety, recombinant human adenovirus has been classified in biosafety level II for agents considered of ordinary potential harm, and you need BL-2 level facility to work with it. It should be noted that cell culture facilities in most institutes are certified as BL-2 level. Wild type, replication competent adenoviruses provoke cold symptoms and strong immune responses in healthy individuals and generally do not cause serious illness. For more information on biosafety levels please visit http://bmbl.od.nih.gov Q. How can I find out whether adenovirus will work well in my cell models? The Adenovirus has a very broad host range; it can infect human and other mammalian cell lines or primary cells, including replicative as well as non-replicative cells. There are in fact very few cell lines that cannot be infected. Some lymphoid cel
