What is the relationship of PNU and Weight to Volume?
PNU is an abbreviation for “Protein Nitrogen Unit”. PNU is a chemical measurement of the amount of nitrogen incorporated in the protein molecules in an allergenic extract. Weight to volume refers to the ratio of the weight of allergen source material to the volume of liquid in which it is extracted. For example, 100 grams of pollen placed into 1,000 mL of extracting liquid yields a 1:10 w/v solution.How is PNU determined?Proteins contain nitrogen – about 17% by weight. A lab procedure called micro-Kjeldahl determines the amount of nitrogen in a sample and the results are expressed as Protein Nitrogen Units (PNU). This is a rough estimate of the total protein in the sample. In some instances, the amount of protein is not directly related to the allergen content or to its allergenicity.Is the amount of protein related to the allergen content?One can not use PNU values to compare the potency of extracts from two or more species. However, there is a relationship between PNU values and alle
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