What is the recommended step size for deconvolution?
Formula for computing optimal step size for deconvolution Table for lenses for air objectives Table for lenses for water objectives Table for lenses for oil objectives Visual Basic Code to compute the above tables The formula used by VolumeScan for calculating the depth of field is: Where: D = depth of field W = wavelength of light (emission) n = refractive index of medium between the lens and the specimen M = magnification of the lens plus optical relay to the camera e = resolution limit of the microscope/camera system The formula can be found on page 48 of Video Microscopy by Inoue’ and Spring, 1997, Plenum Press. (See Books) Other formulae have been proposed by other authors. The refractive index for air is 1.0 and 1.3 for water. The refractive index for oil is printed on the side of the bottle of immersion oil, usually about 1.5. The magnification is the multiple of the lens magnification and the magnification of the relay optics between the lens and the camera, usually 10x. The NA
