What is the recommended RNA extraction method?
Make sure to use one of the commercially available column based RNA extraction kits that are specifically developed for RNA extraction for small RNA studies such as Norgen Biotek, Qiagen miRNeasy or Ambion miRVana kits. Please refer to corresponding manufacturer’s manuals. If you have multiple samples, make sure to use only one type of extraction kit for all the samples of your project. Many laboratories have obtained excellent results from total RNA samples extracted using Trizol methods. However, skills, experiences and sometime sample types may become critical factors in obtaining consistently good sample qualities. According to our statistics, the method has an overall higher failure rate than column-based commercial methods, although the rate varies among different laboratories. If you must use a Trizol method, we recommend modifying precipitation step by doubling the usual isopropanol volume and leaving the RNA at -80C for 10-20 minutes so as to ensure the precipitation of small
