WHAT IS THE RECOMMENDED PROTOCOL FOR GENERATION OF SINGLE-STRAND DNA TARGETS?
The recommended method is based on two amplification steps. First is performed to amplify the double-strand DNA fragment and to incorporate specific tag at one end. The resultant product is cleaned using a PCR product purification kit (e.g. from Qiagen) to remove primers. It is then subjected to a second amplification with only one, reverse specific primer (not the tagged one) for 40 to 50 cycles to produce the single-strand DNA fragment.
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- WHAT IS THE RECOMMENDED PROTOCOL FOR GENERATION OF SINGLE-STRAND DNA TARGETS?
