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What is the recommended procedure for blood collection into the P100 tube?

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What is the recommended procedure for blood collection into the P100 tube?

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• Center tubes in the holder, to prevent sidewall penetration and resultant premature vacuum loss. • Push tube onto non-patient-end (NP-end) of needle in one swift action in order to minimize premature separation of the mechanical separator from the stopper. Hold tube on NP-end during drawing. If separator moves in the tube during blood collection, discard the tube and redraw. • Remove tourniquet as soon as blood appears in tube. Do not allow contents of the tube to contact the stopper or end of the needle during procedure. • Allow vacuum to be exhausted prior to removing the tube from the NP (non-patient) end of the needle. • Mix specimen with tube additives. For optimal mixing, slowly invert tube 8 to 10 times immediately after blood collection before centrifugation.

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