What is the recommended flow rate for each of the steps involved in purification?
In the Capture step, using a flow rate of 0.25 ml/min is recommended for Protein A, Protein G and IMAC resins. For the Purify and Enrich steps, a flow rate of 0.5 mL/min is recommended. Check the supplied specification sheet for details. For Glutathione, it is known that the flow rate of sample over the resin will affect the capture potential; consequently the capture flow rate should be reduced below the recommended 0.25 mL/min. A useful flow rate is 0.06 ml/min for Capture. (See also Q 13 above).
