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What is the recommended culture medium?

April 26, 2017culture medium recommended

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What is the recommended culture medium?

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LB broth is the recommended culture medium, since richer broths lead to higher cell densities, which may clog the QiaPrep filters. Qiagen recommends growing cultures in LB medium containing 10g NaCl, 10g tryptone, and 5g yeast extract per liter to obtain the highest plasmid yield.