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What is the reasonable level of background I can expect on my array?

April 26, 2017array background expect level reasonable

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What is the reasonable level of background I can expect on my array?

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Reasonable background level is one that doesn’t overpower the spots on the array. Good background level is around 1000 with some saturated spots. Excellent background level is 200 with a few saturated spots. To achieve reliable data signal to background ratios should be 2 fold.