What is the purpose of using Bind-Silane in 2-D electrophoresis?
Note: To ensure that glass plates are properly clean after spot picking (and before re-use for spot picking), soak the plates overnight in a solution of alkaline laboratory glassware cleaner. 1. Thoroughly wash the plate to be treated. Take care to remove any gel fragments attached to the plate from previous gels. The careful cleaning of the glass plates before casting is important, to ensure a uniform coating with the Bind-Silane. 2. Dry the plate using a lint-free tissue. 3. Prepare the bind-silane working solution: 8 ml ethanol, 200 l glacial acetic acid, 10 l bind silane, 1.8 ml distilled or de-ionized water. 4. Pipette 2-4 ml (depending on plate size) of the above solution onto the plate and wipe it over with a lint-free tissue. Cover the plate to prevent dust contamination and leave to air dry on the bench for 1.5 hours (minimum 1 hour). Note: If the Bind-Silane is not left for at least 1 hour to dry before the glass plates are assembled for casting, the solution will evaporate o
