What is the purpose of the neuron microfluidic device?
A. The neuron microfluidic device allows researchers to compartmentalize neuronal cultures. The neuron device consists of two culture chambers separated by a microgroove barrier. Neurons are typically loaded on one side of the device. The size of the microgroove barrier allows axons to grow through the barrier while keep the cell bodies (Soma) isolated from the distal chamber. Please note however that the microgrooves will not keep migrating cells from crossing the barrier. In addition high density cultures may result in some cell bodies entering the microgrooves. The neuron device also makes it possible to perform axotomy, cutting axons via vacuum aspiration in the distal chamber and observing their regrowth. The neuron device is also small in size (total volume ~ 600 ul) thus researchers save on the expensive reagents needed for neuronal culture.
