What is the purpose of internal positive controls in real-time PCR?
Internal positive control is another real-time PCR system (template, primers, probe) that is added to the reaction mixture together with the system to detect the target sequence (duplex reaction takes place). Monitoring of the positive control amplification is important to reveal false positive results that may occur due to PCR inhibition. GENERI BIOTECH offers specific internal positive controls or universal control – See here for more information >>.
Related Questions
- I observe extremely high Cts for the immunoprecipitated sample (positive locus) and for my internal controls comparing with the Cts for the input DNA. What does it mean?
- Can the efficiency of the immunoprecipitation (testing the positive and negative controls) be assessed using endpoint PCR instead of qPCR?
- What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
