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What is the purpose of autoclaving in media preparation?

April 26, 2017autoclaving media purpose

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What is the purpose of autoclaving in media preparation?

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Autoclaving is used to kill all micro-organisms that are in the culture. Autoclaving is usually carried out at 121 deg C with steam under 2 atmospheres of pressure. A clean culture free of all micro-organisms is essential as you only want the organism of intrest to grow.