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What is the purpose of adding unlabeled DNA to the binding reaction as a control?

April 26, 2017Binding CONTROL dna purpose REACTION unlabeled

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What is the purpose of adding unlabeled DNA to the binding reaction as a control?

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As a control, unlabeled DNA of the same sequence as the biotin-labeled DNA is added in excess to compete with the biotin-labeled DNA. This results in a decrease or elimination of the “shift”, verifying that the protein-DNA interaction was sequence specific.