What is the procedure for the M30-Apoptosense assay?
The assay is a solid-phase, two-site immunosorbent assay. Samples are reacted with polystyrene wells coated with a catcher monoclonal antibody and, simultaneously with HRP-conjugated monoclonal antibody (M30 antibody) used as a tracer. Following the formation of the solid phase/antigen/labeled antibody sandwich, excess unbound tracer is removed by washing. TMB substrate is added, and the reaction is stopped after an incubation period. The absorbance is measured in a microplate reader. By plotting a standard curve from known concentrations vs. measured absorbance, the amount of M30 antigen in the sample can be calculated. The concentration of the M30 antigen is expressed as units per liter (U/L).
