What is the procedure for Cell Cultures?
Mononuclear cells are isolated from anti coagulated blood by centrifugation in a Cell Separation Tube. The Cell Separation Tube has a polyester gel and Ficoll Hypaque solution which acts as a separation media. During centrifugation the polyester gel moves to form a barrier separating the mononuclear cells and plasma from the red blood cells. The mononuclear cells are collected by pipetting the cell layer and washing with Balanced Salt Solution. The washed mononuclear cells are suspended in growth medium. Epstein Barr Virus and CyclosporinA are added to the cells to set up the cell culture. The cells are grown in an incubator at 37 degrees Celsius and 5 % carbon dioxide.
