What is the principle of TRANSIL assay kits?
A shared feature of all TRANSIL assays is the titration of a constant concentration of the test substance (2 to 50 μM) with different surface areas of lipid or protein, which is achieved by different amounts and sizes of TRANSIL beads per well. Two blank wells are used to determine unspecific binding to the assay plate, hence our estimates of binding or permeability are not confounded by compounds sticking to the plate’s plastic walls.
