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What is the principle of RIA?

April 26, 2017Principle ria

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What is the principle of RIA?

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Unlabelled analyte competes with radiolabelled analyte (usually labelled with I-125) for the limited binding sites available on the antibody molecule made specifically for the analyte of the interest. At the end of reaction labelled analyte bound antibody is separated and measured for the radioactivity. Amount of the radioactivity separated is compared with values of known analyte standards and the concentration of analyte present in the sample is calculated.