What is the principle of PCR-RFLP and mismatch PCR-RFLP method?
Back to the FAQ SNPs recognized by a restriction enzyme in one of the alleles can be genotyped by the PCR-RFLP method. When the SNP does not affect any restriction sequences, so “mismatch (or mismatched) PCR-RFLP” can be employed by introducing an artificial restriction enzyme site. This is achieved using a primer containing additional mismatched bases adjacent to the SNP site. The introduced mismatch can construct a restriction site in one of the allele sequences with the SNP site (shown in the following figure).
