What is the performance with autofluorescent compounds?
By making use of a second, non-fluorescent yeast strain the GreenScreen assay protocol is able to accurately correct for the autofluorescence properties of the majority of test compounds. For highly autofluorescent compounds a patented protocol using fluorescence polarisation can also be used to discriminate between the GFP-derived assay signal and compound autofluorescence, exploiting the high fluorescence anisotropy of GFP.
