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What is the PCR Clean-up Reagent?

clean-up PCR Reagent
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What is the PCR Clean-up Reagent?

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• The reagent is a mixture of two different enzymes: 1) Exo = Exonuclease I which degrades single stranded DNA primers remaining after amplification 2) SAP = Shrimp Alkaline Phosphatase which removes the phosphates from the dNTPs remaining after amplification. • The reagent is sold under a license from USB Corp. • This reagent has been optimized to degrade primers and dNTPs as recommended in AcycloPrime system. • The reagent is substantially different than Exo-SAP-IT, USB Catalog Number 78201.

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