What is the OriGene guarantee on the shRNA expression plasmids?
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. The shRNA expression plasmid vectors have been validated in gene silencing studies against green fluorescent protein, luciferase, red fluorescence protein and human HER2 oncogene. If none of the four OriGene constructs produces at least 70% gene expression knock-down, our scientists will work with you to pinpoint the problem and, if it is determined that our constructs are at fault, OriGene will replace your HuSH constructs with another set of four (4) new constructs against that target gene, free of charge.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the
