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What is the optimal selection for the mismatch base number and position?

April 26, 2017base mismatch optimal position selection

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What is the optimal selection for the mismatch base number and position?

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Back to the FAQ The SNP Cutter introduces one base mismatched in primer to create an artificial enzyme recognition site. Users can decide how many nucleotides at the primer 3′ end should be free of mismatch. The default value is 2. It is not recommend to have the mismatch at the last two nucleotides.