What is the optimal DNA concentration to be used on a Elchrom gel?
A12: Loading too much DNA per lane affects the migration of individual fragments rather substantially (overloading). Furthermore, the optimal concentration of DNA and therefore resolution also depends on the detection method: for Ethidium Bromide staining, the optimal concentration of dsDNA is between 2 and 20 ng per band; for SYBR staining, between 0.4 and 4 ng per band.
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