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What is the optimal concentration and DNA quality for the best genotyping ?

April 26, 2017best concentration dna Genotyping optimal quality

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What is the optimal concentration and DNA quality for the best genotyping ?

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Sequenom recommends an A260/A280 ratio of 1.7 to 2.0, and 5 to 10 ng/ul of DNA for each sample in a multiplex. They found that the reactions work with up to 50ng DNA, above that performance degrades. We use 10 ng per multiplex.