What is the minimum starting amount of total RNA that can be used in the RampUP protocol?
Best results can be obtained when using at least 1ng, however, as little as 10pg has been used successfully. The amplification yield of RampUP will vary from sample to sample due to differences in mRNA content and quality. For example, 20ng of a very intact RNA sample (Mouse Brain Total RNA, Ambion cat. no. AM7812) may yield 100µg of senseRNA, whereas 20ng of an FFPE sample may yield 20µg senseRNA or more. Therefore, Genisphere recommends using 1-25ng of intact RNA or 20-25ng of FFPE RNA with RampUP.
