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What is the minimum/maximum reaction volume I should be using in the ABI PRISM® 7000 and ABI Prism® 7900HT?

April 26, 2017Abi maximum minimum REACTION volume

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What is the minimum/maximum reaction volume I should be using in the ABI PRISM® 7000 and ABI Prism® 7900HT?

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For 96 wells plate, the minimum reaction volume is 25l for real time PCR and for Allelic Discrimination plate reads. For 384 wells plate, the minimum reaction volume is 10l for real time PCR and 5l for Allelic Discrimination plate reads. The maximum volume for 96 wells reaction is 50l and for 384 wells reaction is 20l.