What is the minimum amount of total RNA I can use for each Affymetrix GeneChip expression array?
We currently recommend submitting 15ug of total RNA for each Affy experiment. Although we use 5ug of total RNA for each eukaryotic expression the extra amount allows us to assess RNA quality and quantity. Reducing the amount of starting total RNA may result in a subsequent decrease in sensitivity. For those researchers with limited amounts of starting material, a protocl is available from Affymetrix for performing experiments using as little as 100ng of sample.
