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What is the minimum amount of DNA required to perform a NimbleGen recommended MeDIP experiment?

April 26, 2017dna experiment medip minimum NimbleGen perform recommended

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What is the minimum amount of DNA required to perform a NimbleGen recommended MeDIP experiment?

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We recommend starting with 5μg high-quality genomic DNA. However, even smaller amount DNA can be used (1μg or even 200ng). The IP process generally yields 5-10% of the original starting DNA, and you can amplify your IP-ed DNA using whole genome amplification (WGA; kit available from Sigma) in order to obtain at least 4μg of DNA.