Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What is the method you recommend for screening the stable clones and is there a pair of primers that can be used for screening them by PCR?

April 26, 2017clones method pair PCR Primers recommend screening STABLE Used

0

Posted

What is the method you recommend for screening the stable clones and is there a pair of primers that can be used for screening them by PCR?

1 Answer

0

Posted

Puromycin resistant clones may be screened via qRT-PCR using primers designed against PAC or the target gene of interest.