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What is the measurement theory behind the M30-Apoptosense assay?

April 26, 2017assay m30-apoptosense measurement Theory

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What is the measurement theory behind the M30-Apoptosense assay?

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During apoptosis, caspases will cleave various cellular proteins. In epithelial cells, one of those proteins is the intermediate filament cytokeratin 18. The assay is an ELISA for the global determination of apoptosis in epithelial cells. The M30 antibody is a mouse monoclonal of the IgG2b subtype and recognizes a neo-epitope in the C-terminal domain of cytokeratin 18 (amino acids 387 – 396), which is exposed after cleavage by caspases during apoptosis. Several caspases appear to be capable of cleaving CK 18 and expose the neo-epitope, e.g. caspase 3, 6, 7 and 9.