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What is the maximum number of eukaryotic cells which can be used for an electroporation?

April 26, 2017cells electroporation eukaryotic maximum Used

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What is the maximum number of eukaryotic cells which can be used for an electroporation?

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We recommend using a cell concentration of 1 x 106 cells/ml. In this range, the electric field can still efficiently influence the cells. At higher cell densities (>3 x 106) homogeneous field conditions are no longer ensured, i.e. the cells are no longer subjected to a uniform electric field. This can lead e.g. to a decrease in the transfection rate.