What is the maximum load for my SWxl size exclusion column (7.8mm ID x 30cm)?
When it comes to analyzing how much of a component is present in a sample, we want to make sure to work within the linear range of the distribution isotherm, the shape of which describes the relationship between sample concentration in mobile and stationary phases. In preparative chromatography, where the goal is to isolate one of more sample components at a certain level of purity, we can always inject more as long as the component of interest can be collected at the required level of purity. Thus, the amount of sample that can be injected on a column is arbitrarily defined as the mass of sample injected that causes the efficiency of the peak to decrease by no more that 10%. Again, from a practical point of view, as long as the compound of interest is well separated from its nearest contaminant, we can always inject more as long as we obtain at least the desired level of purity. Obviously, the more packing material in the column (i.e., the larger the column volume), the more sample ma
