What is the lowest amount of sample needed to use the NCode™ Rapid miRNA labeling system and array?
When using the NCode™ Amplification Kit, at least 50 ng of total RNA is needed to run one slide in a single color. If the NCode™ Amplification Kit is not used, then approximately 250ng – 5μg of total RNA is needed to run one slide in a single color or to run two slides with different colors (dye-swap). NOTE: If less than 250 ng of starting sample is available, then we recommend the using the NCode™ Amplification Kit. For starting sample less than 500 ng, amplification is not required; however, it should be considered where there is interest in investigating miRNAs that are expressed a low levels and replicates are desired. For the original NCode™ miRNA Labeling sytem, 10 µg of total RNA (or equivalent cells or tissue) was recommended and yields approximately 200 -600 ng of small RNA, depending on the sample.
Related Questions
- NCode™ Rapid miRNA labeling system indicates total RNA should be used as starting material. Will I obtain better results if miRNA is used?
- What is the lowest amount of sample needed to use the NCode™ Rapid miRNA labeling system and array?
- Is the NCode miRNA Rapid Labeling System compatible with the non-coding array?
