What is the general process of 2D DIGE?
Please click for an overview of the 2D DIGE work process. Protein samples are extracted from the cells or tissues, and protein concentration is measured and adjusted. The same amount of each protein sample is labeled with size and charge-matched minimal fluorescent CyDye. Up to three fluorescently labeled protein samples (for example, a normal control, a disease sample and a treatment sample) can be mixed and loaded onto the same electrophoresis gel. Most of our clients start with an analytical gel, on which small amount of labeled proteins are loaded (25-30 ug per sample, total protein is about 75-90 ug). This ensures that maximal resolution is achieved. The analytical images are excellent for publication and grant application purposes.
