What is the function of restriction enzymes that naturally occur in bacteria cells?
Restiriction enzymes, or endonucleases, splice (cut) apart two different sites of the nucleotide sequence on foreign DNA resulting in two different pieces of DNA for a gene of interest to be inserted. This usually occurs in bacteria such as E. coli and such. Once the two cuts have been made (one at 5′ and one at 3′) there remains the open ends of the DNA called ‘sticky ends’. THis is where the gene of interest is inserted.
