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What is the function of nuclear preparation buffer? When do you add the protease inhibitor?

April 26, 2017buffer function inhibitor nuclear protease

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What is the function of nuclear preparation buffer? When do you add the protease inhibitor?

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The nuclear preparation buffer is a hypotonic salt solution that serves to swell the cells and facilitate the release of nuclei during the subsequent homogenization step. The protease inhibitor cocktail should be added just before use.