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What is the function of methylene blue in spectrophotometry?

April 26, 2017BLUE function methylene spectrophotometry

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What is the function of methylene blue in spectrophotometry?

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Methylene blue is used as an indicator for the presence of certain Ions such as the presence of HS – ion. spectrophotometry is used once the methylene blue has reacted to determine the quantity of said ion based on the intensity of blue that the reacion produces. Using a spectrophotometer you can determine the intensity and compare it with a set scale that has been calibrated with known concentrations of this ion.