What is the fidelity of the synthesized peptide?
The protocol incorporates an acetylation step at the end of each cycle to “cap” any unreacted free amines with acetic anhydride. This prevents them from coupling to any subsequent amino acids and virtually eliminates the synthesis of deletion sequences. The purity of the peptides synthesized varies for each peptide and is dependent upon sequence and length. Our analyses have determined that peptide purity is typically >70% for average 6-15 mers (by HPLC and mass spectroscopy).
