What is the EDF option?
EDF stands for Extended Depth of Field and it allows the ImageStreamX to keep the entire cell in focus rather than just a single plane of focus as in conventional microscopy. EDF is extremely useful for assays that rely on the counting of spots, such as fluorescence in situ hybridization, but it is also generally useful. EDF produces imagery similar to a maximum projection of a confocal image stack, but it imposes no speed or sensitivity penalty compared to the standard ImageStreamX optical system. EDF also allows faster operation of the instrument by running a wider fluid core and improves data analysis and consistency by ensuring uniform focus for virtually all the cells in the data file.
