What is the dynamic range of the NCode™ Multi-Species miRNA Microarray?
When starting with 10 ug of total RNA for miRNA enrichment for the original labeling system,, we were able to detect down to 0.3 fmol (corresponding to hundreds of copies) with static hybridization. Incubation in the MAUI hybridization station further improves the detection sensitivity. We have not run into a case of saturating the detectors due to having too high a concentration of miRNA. When starting with 500 ng -5 ug total RNA for the NCode Rapid miRNA labeling system, we were able to detect attamolar levels of miRNA corresponding to 2-10 copies per cell with the the MAUI hybridization station. Lower starting amounts of total RNA may be used (down to 100ng has been tested) but keep in mind that lower expressed miRNAs may be lost with reduction of starting material amount.
