What is the difference in protocol for suspension cells and adherent cells?
We have tailored and optimized a protocol for suspension cells that is detailed in the instruction manual. Briefly, during the incubation step when MATra-A Reagent/nucleic acid complexes or MA Lipofection Enhancer/nucleic acid/transfection reagent (or virus) complexes will form, dilute the cells to be transfected to 5×105 – 1×106 per ml in medium (with or without serum or supplement) and perform one of the following options to sediment the cells at the bottom of the culture dish thus making them susceptible to MATra. Option 1: Mix cell suspension with 30 µl of MATra-S Immobilizer per 1ml of cell suspension. a. Incubate for 10-15 minutes. b. Distribute cells to the culture dish placed upon the magnet plate (volume of culture medium containing cells depends on the culture dish size; see transfection volume as suggested in the protocol). c. Incubate for 15 minutes. d. Add the mixture containing the nucleic acid/MATra-A Reagent complexes to the cells while keeping the culture plate on the
